By Reinhard Neubert (Editor), Hans-Hermann Ruttinger (Editor)
This quantity provides breakthroughs and methods in affinity capillary electrophoresis to degree and be sure the physicochemical and thermodynamic parameters of drug compounds. It discusses recommendations to discover and symbolize interactions to facilitate advancements in managed drug supply and concentrating on.
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Additional resources for Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs)
Capillary isoelectric focusing is based on the migration of zwitterionic analytes in a continuous pH gradient. Therefore, the capillary is ﬁlled with the sample and a mixture of ampholytes having pI values that span the desired pH range, for example, pH 3–10. , phosphoric acid). Upon application of an electric ﬁeld, the ampholytes move to a region where they become uncharged, thus forming a continuous pH gradient. In this gradient the sample proteins are focused into a volume element in the capillary where the pH equals their pI value.
11) yields S ϩ kЈи SL Ϫ S Ϫ kЈи s 1 ϩ kЈ Ϫ S kЈ = =Y SL Ϫ S 1 ϩ kЈ Ϫ S = (12) (13) Replacing kЈ with Eq. (5) gives Ϫ S KA и[L] = SL Ϫ S 1 ϩ KA и[L] (14) or = S ϩ KA и[L]и SL 1 ϩ KA и[L] (15) A typical mobility plot calculated with Eq. (15) is given in Fig. 1 for KA = 105 L/mol. All mobilities are between S and SL . 26 Ru¨ttinger Fig. 1 Typical binding curve using Eq. (15). Nonlinear regression analysis of a plot of or Y against [L] provides the association constant KA , and the electrophoretic mobility of the pure complex SL from the experimental data.
Correctly if too many independent parameters are involved. Due to the statistical errors of the observed mobility data and other imponderabilities, regression analysis fails. In some cases the equations can be simpliﬁed. If binding is highly cooperative, then the concentrations of complexes with lower stoichiometry are very small and all bonded substrate is attributed to the complex of the highest stoichiometry: KA = [SLn ] [S]и[L]n (29) In this case only a simple adaptation of Eq. (5) is needed and inserting kЈ into Eq.
Affinity Capillary Electrophoresis in Pharmaceutics and Biopharmaceutics (Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs) by Reinhard Neubert (Editor), Hans-Hermann Ruttinger (Editor)